Stability of transgenes in trees: expression of two reporter genes in poplar over three field seasons.
Identifieur interne : 003481 ( Main/Exploration ); précédent : 003480; suivant : 003482Stability of transgenes in trees: expression of two reporter genes in poplar over three field seasons.
Auteurs : Jingyi Li [États-Unis] ; Amy M. Brunner ; Richard Meilan ; Steven H. StraussSource :
- Tree physiology [ 0829-318X ] ; 2009.
Descripteurs français
- KwdFr :
- ADN bactérien (MeSH), ADN recombiné (MeSH), Arbres (génétique), Arbres (métabolisme), Composés organiques du phosphore (MeSH), Dosage génique (MeSH), Expression des gènes (MeSH), Extinction de l'expression des gènes (MeSH), Gènes de plante (MeSH), Gènes rapporteurs (MeSH), Herbicides (MeSH), Instabilité du génome (MeSH), Organogenèse (MeSH), Populus (génétique), Populus (métabolisme), Protéines à fluorescence verte (génétique), Régulation de l'expression des gènes végétaux (MeSH), Résistance aux herbicides (génétique), Statistique non paramétrique (MeSH), Transgènes (MeSH).
- MESH :
- génétique : Arbres, Populus, Protéines à fluorescence verte, Résistance aux herbicides.
- métabolisme : Arbres, Populus.
- ADN bactérien, ADN recombiné, Composés organiques du phosphore, Dosage génique, Expression des gènes, Extinction de l'expression des gènes, Gènes de plante, Gènes rapporteurs, Herbicides, Instabilité du génome, Organogenèse, Régulation de l'expression des gènes végétaux, Statistique non paramétrique, Transgènes.
English descriptors
- KwdEn :
- DNA, Bacterial (MeSH), DNA, Recombinant (MeSH), Gene Dosage (MeSH), Gene Expression (MeSH), Gene Expression Regulation, Plant (MeSH), Gene Silencing (MeSH), Genes, Plant (MeSH), Genes, Reporter (MeSH), Genomic Instability (MeSH), Green Fluorescent Proteins (genetics), Herbicide Resistance (genetics), Herbicides (MeSH), Organogenesis (MeSH), Organophosphorus Compounds (MeSH), Populus (genetics), Populus (metabolism), Statistics, Nonparametric (MeSH), Transgenes (MeSH), Trees (genetics), Trees (metabolism).
- MESH :
- chemical , genetics : Green Fluorescent Proteins.
- chemical : DNA, Bacterial, DNA, Recombinant, Herbicides, Organophosphorus Compounds.
- genetics : Herbicide Resistance, Populus, Trees.
- metabolism : Populus, Trees.
- Gene Dosage, Gene Expression, Gene Expression Regulation, Plant, Gene Silencing, Genes, Plant, Genes, Reporter, Genomic Instability, Organogenesis, Statistics, Nonparametric, Transgenes.
Abstract
High stability of transgene expression is essential for functional genomics studies using transformation approaches and for application of genetic engineering to commercial forestry. We quantified expression of two reporter genes, green fluorescent protein (GFP) and the herbicide bialaphos resistance gene (BAR), in 2256 transgenic poplar trees derived from 404 primary events, and in 106 in vitro-redifferentiated subevents, over 3 years in the greenhouse and in the field. No gene silencing (complete breakdown of expression) was observed for GFP or BAR expression in any of the primary transgenic events during the course of the study. Transgenic cassettes were physically eliminated in four subevents (2.5%) derived from three different primary events during re-organogenesis. Transgene copy number was positively correlated with transgene expression level; however, a majority of transformants (85%) carried single-copy transgenes. About one-third of the events containing two-copy inserts had repeats formed at the same chromosomal position, with direct repeats being the main type observed (87%). All events containing more than two transgene copies showed repeat formation at least at one locus, with direct repeats again dominant (77%). Loci with two direct repeats had substantially greater transgene expression level than other types of two-copy T-DNA configurations, but insert organization was not associated with stability of transgene expression. Use of the poplar rbcS promoter, which drove BAR in the transgenic constructs, had no adverse effect on transgene expression levels or stability compared with the heterologous CaMV 35S promoter, which directed GFP expression.
DOI: 10.1093/treephys/tpn028
PubMed: 19203955
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Stability of transgenes in trees: expression of two reporter genes in poplar over three field seasons.</title><author><name sortKey="Li, Jingyi" sort="Li, Jingyi" uniqKey="Li J" first="Jingyi" last="Li">Jingyi Li</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331-5752, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331-5752</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Brunner, Amy M" sort="Brunner, Amy M" uniqKey="Brunner A" first="Amy M" last="Brunner">Amy M. Brunner</name></author><author><name sortKey="Meilan, Richard" sort="Meilan, Richard" uniqKey="Meilan R" first="Richard" last="Meilan">Richard Meilan</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19203955</idno><idno type="pmid">19203955</idno><idno type="doi">10.1093/treephys/tpn028</idno><idno type="wicri:Area/Main/Corpus">003653</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003653</idno><idno type="wicri:Area/Main/Curation">003653</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003653</idno><idno type="wicri:Area/Main/Exploration">003653</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Stability of transgenes in trees: expression of two reporter genes in poplar over three field seasons.</title><author><name sortKey="Li, Jingyi" sort="Li, Jingyi" uniqKey="Li J" first="Jingyi" last="Li">Jingyi Li</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331-5752, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331-5752</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Brunner, Amy M" sort="Brunner, Amy M" uniqKey="Brunner A" first="Amy M" last="Brunner">Amy M. Brunner</name></author><author><name sortKey="Meilan, Richard" sort="Meilan, Richard" uniqKey="Meilan R" first="Richard" last="Meilan">Richard Meilan</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></analytic><series><title level="j">Tree physiology</title><idno type="ISSN">0829-318X</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Bacterial (MeSH)</term><term>DNA, Recombinant (MeSH)</term><term>Gene Dosage (MeSH)</term><term>Gene Expression (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Gene Silencing (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Genomic Instability (MeSH)</term><term>Green Fluorescent Proteins (genetics)</term><term>Herbicide Resistance (genetics)</term><term>Herbicides (MeSH)</term><term>Organogenesis (MeSH)</term><term>Organophosphorus Compounds (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Statistics, Nonparametric (MeSH)</term><term>Transgenes (MeSH)</term><term>Trees (genetics)</term><term>Trees (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (MeSH)</term><term>ADN recombiné (MeSH)</term><term>Arbres (génétique)</term><term>Arbres (métabolisme)</term><term>Composés organiques du phosphore (MeSH)</term><term>Dosage génique (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Extinction de l'expression des gènes (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Gènes rapporteurs (MeSH)</term><term>Herbicides (MeSH)</term><term>Instabilité du génome (MeSH)</term><term>Organogenèse (MeSH)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines à fluorescence verte (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Résistance aux herbicides (génétique)</term><term>Statistique non paramétrique (MeSH)</term><term>Transgènes (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Recombinant</term><term>Herbicides</term><term>Organophosphorus Compounds</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Herbicide Resistance</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arbres</term><term>Populus</term><term>Protéines à fluorescence verte</term><term>Résistance aux herbicides</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Dosage</term><term>Gene Expression</term><term>Gene Expression Regulation, Plant</term><term>Gene Silencing</term><term>Genes, Plant</term><term>Genes, Reporter</term><term>Genomic Instability</term><term>Organogenesis</term><term>Statistics, Nonparametric</term><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN bactérien</term><term>ADN recombiné</term><term>Composés organiques du phosphore</term><term>Dosage génique</term><term>Expression des gènes</term><term>Extinction de l'expression des gènes</term><term>Gènes de plante</term><term>Gènes rapporteurs</term><term>Herbicides</term><term>Instabilité du génome</term><term>Organogenèse</term><term>Régulation de l'expression des gènes végétaux</term><term>Statistique non paramétrique</term><term>Transgènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High stability of transgene expression is essential for functional genomics studies using transformation approaches and for application of genetic engineering to commercial forestry. We quantified expression of two reporter genes, green fluorescent protein (GFP) and the herbicide bialaphos resistance gene (BAR), in 2256 transgenic poplar trees derived from 404 primary events, and in 106 in vitro-redifferentiated subevents, over 3 years in the greenhouse and in the field. No gene silencing (complete breakdown of expression) was observed for GFP or BAR expression in any of the primary transgenic events during the course of the study. Transgenic cassettes were physically eliminated in four subevents (2.5%) derived from three different primary events during re-organogenesis. Transgene copy number was positively correlated with transgene expression level; however, a majority of transformants (85%) carried single-copy transgenes. About one-third of the events containing two-copy inserts had repeats formed at the same chromosomal position, with direct repeats being the main type observed (87%). All events containing more than two transgene copies showed repeat formation at least at one locus, with direct repeats again dominant (77%). Loci with two direct repeats had substantially greater transgene expression level than other types of two-copy T-DNA configurations, but insert organization was not associated with stability of transgene expression. Use of the poplar rbcS promoter, which drove BAR in the transgenic constructs, had no adverse effect on transgene expression levels or stability compared with the heterologous CaMV 35S promoter, which directed GFP expression.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19203955</PMID><DateCompleted><Year>2009</Year><Month>04</Month><Day>15</Day></DateCompleted><DateRevised><Year>2009</Year><Month>02</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0829-318X</ISSN><JournalIssue CitedMedium="Print"><Volume>29</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Stability of transgenes in trees: expression of two reporter genes in poplar over three field seasons.</ArticleTitle><Pagination><MedlinePgn>299-312</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpn028</ELocationID><Abstract><AbstractText>High stability of transgene expression is essential for functional genomics studies using transformation approaches and for application of genetic engineering to commercial forestry. We quantified expression of two reporter genes, green fluorescent protein (GFP) and the herbicide bialaphos resistance gene (BAR), in 2256 transgenic poplar trees derived from 404 primary events, and in 106 in vitro-redifferentiated subevents, over 3 years in the greenhouse and in the field. No gene silencing (complete breakdown of expression) was observed for GFP or BAR expression in any of the primary transgenic events during the course of the study. Transgenic cassettes were physically eliminated in four subevents (2.5%) derived from three different primary events during re-organogenesis. Transgene copy number was positively correlated with transgene expression level; however, a majority of transformants (85%) carried single-copy transgenes. About one-third of the events containing two-copy inserts had repeats formed at the same chromosomal position, with direct repeats being the main type observed (87%). All events containing more than two transgene copies showed repeat formation at least at one locus, with direct repeats again dominant (77%). Loci with two direct repeats had substantially greater transgene expression level than other types of two-copy T-DNA configurations, but insert organization was not associated with stability of transgene expression. Use of the poplar rbcS promoter, which drove BAR in the transgenic constructs, had no adverse effect on transgene expression levels or stability compared with the heterologous CaMV 35S promoter, which directed GFP expression.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jingyi</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331-5752, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brunner</LastName><ForeName>Amy M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Meilan</LastName><ForeName>Richard</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>Steven H</ForeName><Initials>SH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>12</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004274">DNA, Recombinant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006540">Herbicides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009943">Organophosphorus Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C036483">T-DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>35597-43-4</RegistryNumber><NameOfSubstance UI="C003122">bialaphos</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004274" MajorTopicYN="N">DNA, Recombinant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018628" MajorTopicYN="N">Gene Dosage</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="Y">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="Y">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="Y">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042822" MajorTopicYN="Y">Genomic Instability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053209" MajorTopicYN="N">Herbicide Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006540" MajorTopicYN="N">Herbicides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038081" MajorTopicYN="N">Organogenesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009943" MajorTopicYN="N">Organophosphorus Compounds</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018709" MajorTopicYN="N">Statistics, Nonparametric</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="Y">Transgenes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>2</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>2</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>4</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19203955</ArticleId><ArticleId IdType="pii">tpn028</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpn028</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oregon</li></region></list><tree><noCountry><name sortKey="Brunner, Amy M" sort="Brunner, Amy M" uniqKey="Brunner A" first="Amy M" last="Brunner">Amy M. Brunner</name><name sortKey="Meilan, Richard" sort="Meilan, Richard" uniqKey="Meilan R" first="Richard" last="Meilan">Richard Meilan</name><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></noCountry><country name="États-Unis"><region name="Oregon"><name sortKey="Li, Jingyi" sort="Li, Jingyi" uniqKey="Li J" first="Jingyi" last="Li">Jingyi Li</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003481 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003481 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19203955
|texte= Stability of transgenes in trees: expression of two reporter genes in poplar over three field seasons.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19203955" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |